Despite effective treatment of existing
tumors, patients with
bladder cancer remain at risk of developing new
tumors. Effective
immunotherapy may lower that risk. To test this hypothesis, mice that had survived
transitional cell carcinoma (MBT2)
transplantation with the aid of bacillus Calmette-Guerin
immunotherapy were randomized and tested for long term protective immunity against bladder
carcinoma. Fifty-one
tumor-free mice that had survived
tumor challenge 10 to 15 months previously were randomized into 3 groups to receive intradermal
tumor .noculation and intraperitoneal
levamisole, intralesional Tice strain bacillus Calmette-Guerin, or intralesional saline. Fifteen previously unchallenged animals also received
tumor and intralesional saline. All 3 groups of survivors had less
tumor growth (p less than 0.01) than nonsurviving controls. Even among survivors, additional bacillus Calmette-Guerin immunization, but not
levamisole treatment, significantly inhibited
tumor growth (p less than 0.01). A 2nd experiment compared 22 nonimmune mice, 21 mice preimmunized intravenously with 300 micrograms of bacillus Calmette-Guerin cell walls, and 18 mice that had survived MBT2 by 8 months after live bacillus Calmette-Guerin treatment. Nonimmune and survivor groups were randomly subdivided into saline or treatment groups. Cell wall-preimmunized mice were divided into matching groups according to footpad response to purified
protein derivative. The cell-wall preimmunized and nonimmune mice received the
immunostimulant P3+Re-
glycolipid or the carrier
solution alone. The group of survivors received either intralesional saline or live bacillus Calmette-Guerin. Both bacillus Calmette-Guerin and saline-treated groups had significantly less
tumor growth (p less than 0.001) than nonsurviving controls. Animals treated with P3-Re-glycolipid (with or without preimmunization with cell wall) did not differ from nonsurviving control. Footpad response to purified
protein derivative did not correlate with
tumor growth in these mice. Our results suggest that intralesional bacillus Calmette-Guerin
immunotherapy can afford long term protection from transplanted
bladder cancer, and that live bacillus Calmette-Guerin is superior to
levamisole and P3 +
Re-glycolipid + bacillus Calmette-Guerin cell walls in the treatment of
bladder cancer.