Serological diagnosis of deep Staphylococcus aureus infections by enzyme-linked immunosorbent assay (ELISA) for staphylococcal hemolysins and teichoic acid.

An enzyme-linked immunosorbent assay (ELISA) was used with a purified alpha-toxin preparation to measure the serum IgG, IgM and IgA response in staphylococcal septicaemia and endocarditis. ELISA for IgG antibodies against alpha-toxin was found to be more sensitive than the neutralization test (ASTA). IgM and IgA antibody determination was found to be of limited diagnostic value. A correlation between IgG antibodies to alpha-toxin and purified beta-toxin was found in ELISA, although antibody determination to beta-toxin was a less sensitive diagnostic method. The highest diagnostic sensitivity in deep staphylococcal infections was obtained by parallel performance of ELISA to alpha-toxin and purified teichoic acid. By this approach, 32/35 (91%) patients with endocarditis, 12/14 (86%) with complicated septicaemia and 15/22 (68%) with uncomplicated septicaemia showed increased titres in samples drawn between days 7-30 of disease. Diagnostic sensitivity was further increased to 31/32 (97%) positive patients, when paired or multiple samples from patients with septicaemic staphylococcal disease were analysed.
AuthorsM Granström, I Julander, R Möllby
JournalScandinavian journal of infectious diseases. Supplementum (Scand J Infect Dis Suppl) Vol. 41 Pg. 132-9 ( 1983) ISSN: 0300-8878 [Print] SWEDEN
PMID6379858 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • Bacterial Toxins
  • Hemolysin Proteins
  • Teichoic Acids
  • staphylococcal alpha-toxin
  • Sphingomyelin Phosphodiesterase
  • hlb protein, Staphylococcus aureus
  • Antibodies, Bacterial (analysis)
  • Bacterial Toxins (immunology)
  • Endocarditis, Bacterial (diagnosis, immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Furunculosis (diagnosis, immunology)
  • Hemolysin Proteins (immunology)
  • Humans
  • Immunoenzyme Techniques
  • Osteomyelitis (diagnosis, immunology)
  • Sepsis (diagnosis, immunology)
  • Sphingomyelin Phosphodiesterase
  • Staphylococcal Infections (diagnosis, immunology)
  • Staphylococcus aureus (immunology)
  • Teichoic Acids (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!

Choose Username:
Verify Password: