Immunopharmacologic profiles of a thiol protease inhibitor, L-trans-dicyclohexyl epoxysuccinate.

Abstract

Ep-1, L-trans-Dicyclohexyl epoxysuccinate, is a synthetic and specific inhibitor of thiol proteases. The effects of this inhibitor on some immunological parameters were examined in normal and immunity-impaired mice and rats. In the cultures of splenocytes obtained from the mice treated with Ep-1, it enhanced the lymphocyte blast transformation induced by both suboptimal and optimal concentrations of concanavalin A (Con A) and Lens culinaris (LC). The in vivo administration of Ep-1 caused a depression of the plaque forming cells (PFC) for sheep red blood cell (SRBC) and enhanced the delayed-type hypersensitivity (DTH) for bovine serum albumin (BSA) as well as mixed lymphocytes cultures (MLC). Furthermore, Ep-1 demonstrated a preventive effect on adjuvant arthritic rats. The relevance of immunological regulation and the mode of action of Ep-1 as a thiol protease inhibitor are discussed in these findings.

• Authors: T Amamoto, T Okazaki, T Komurasaki, K Oguma, M Tamai, K Hanada, S Omura
• Journal: Japanese journal of pharmacology (Jpn J Pharmacol) Vol. 34 Issue 3 Pg. 335-42 (Mar 1984) ISSN: 0021-5198 [Print] Japan
• PMID: 6374218 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Cyclohexanes
• Mitogens
• Protease Inhibitors
• dicyclohexyl 2,3-epoxysuccinate

Topics

• Adjuvants, Immunologic
• Animals
• Arthritis, Experimental (immunology)
• Cyclohexanes (pharmacology)
• Female
• Hemolytic Plaque Technique
• Hypersensitivity, Delayed (immunology)
• Lymphocyte Activation (drug effects)
• Lymphocytes (drug effects)
• Male
• Mice
• Mice, Inbred C3H
• Mice, Inbred C57BL
• Mitogens (pharmacology)
• Protease Inhibitors (pharmacology)
• Rats
• Rats, Inbred Strains
• Spleen (cytology)