Differential agglutination by soybean agglutinin of human leukemia and neuroblastoma cell lines: potential application to autologous bone marrow transplantation.

Abstract

Normal human bone marrow cells were mixed with radioactively labeled tumor cells from different leukemia and neuroblastoma cell lines, and the cell mixtures were separated by differential agglutination with soybean agglutinin. It is shown that the cell fraction unagglutinated by soybean agglutinin, which was previously found to be capable of reconstituting the hematopoietic system of lethally irradiated recipients, can be purged of tumor cells with varying efficiency depending on the tumor cell expression of soybean agglutinin receptors as detected by flow cytofluorimetry with fluoresceinated soybean agglutinin.

• Authors: Y Reisner
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 80 Issue 21 Pg. 6657-61 (Nov 1983) ISSN: 0027-8424 [Print] United States
• PMID: 6356131 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Lectins
• Plant Lectins
• Soybean Proteins
• soybean lectin

Topics

• Animals
• Antigens, Neoplasm (analysis)
• Bone Marrow Cells
• Bone Marrow Transplantation
• Cell Line
• Cross Reactions
• Humans
• Immunologic Techniques
• Immunotherapy
• Lectins
• Leukemia (therapy)
• Leukemia, Experimental (immunology)
• Neuroblastoma (immunology)
• Plant Lectins
• Soybean Proteins