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Systemic senile amyloid in senescence-accelerated mice. A unique fibril protein demonstrated in tissues from various organs by the unlabeled immunoperoxidase method.

Abstract
Tissue sections of senescence-accelerated mice (SAM) and of mice with normal aging characteristics (R series) were studied using a peroxidase-antiperoxidase (PAP) method with specific antisera against a unique amyloid fibril protein (AS/SAM) and against murine protein AA. Anti-AS/SAM antisera reacted with amyloid tissues of SAM and aged R series mice but not with normal tissues or amyloid tissues containing protein AA. Reactivity of this and serum could be abrogated by absorption with purified AS/SAM. Amyloid deposits in liver, kidney, spleen, gastrointestinal tract, lung, heart, gonads, pancreas, salivary glands, adrenal, thyroid, skin epineurium, and blood vessels were positive for AS/SAM in both the SAM and R series. Brain and bone medulla, however, were not stained. The amyloid deposited in gonads, papillary layer of dermis, and epineurium was exclusively AS/SAM. The amyloid observed in most of the P-1 series mice was AS/SAM. In the P-2 series and R series, protein AA frequently coexisted with AS/SAM as demonstrated using a double staining method. Each amyloid localized preferentially in the liver, spleen, and gastrointestinal tract.
AuthorsK Higuchi, A Matsumura, A Honma, S Takeshita, K Hashimoto, M Hosokawa, K Yasuhira, T Takeda
JournalLaboratory investigation; a journal of technical methods and pathology (Lab Invest) Vol. 48 Issue 2 Pg. 231-40 (Feb 1983) ISSN: 0023-6837 [Print] United States
PMID6337302 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amyloid
  • Immune Sera
  • Serum Amyloid A Protein
  • amyloid fibril protein AS-SAM
Topics
  • Aging
  • Amyloid (isolation & purification)
  • Amyloidosis (pathology)
  • Animals
  • Female
  • Immune Sera (immunology)
  • Immunoenzyme Techniques
  • Male
  • Mice
  • Mice, Inbred Strains
  • Rabbits (immunology)
  • Serum Amyloid A Protein (metabolism)

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