Five antigens on human T cells detected by mouse monoclonal antibodies.

Abstract

Five antigen systems were defined by the monoclonal antibodies (MoAb) produced against mature T cells. The antigens recognized were grouped into two categories based on the antigen distribution on T cells. (a) Tp 120 [mol. wt 120 kilodaltons (120kD)] and Tp40 (40kD), these are on most peripheral T cells, but not on any other cell lineages, i.e. pan-T antigen. (b) Ts32 (32kD), Ts145 (145kD) and TsA (not determined), these antigens are present only on certain populations of peripheral T cells, i.e., T subset antigen. Among these five, Ts145 and TsA are probably novel T cell antigens. Cell surface phenotypes of leukaemias and lymphomas were typed with these MoAb. Ia like antigen negative, null cell type acute lymphocytic leukaemia (Ia- null ALL) are Tp40+, suggesting that this type of ALL belongs to a T cell lineage. T cell ALL (T-ALL) and lymphoblastic lymphoma (LL) were both Tp40+, Ts32+, TsA+ and a half of the cases were Tp120+, but the expression of Tp40 was stronger on LL cells. Mature T cell (T2) lymphoma and adult T cell leukaemia (ATL) were Tp120+, TsA+, while Tp40 was weakly expressed on only one third of the cases. These MoAb were found to be useful to estimate the origin of various T cell malignancies.

• Authors: I Tsuge, R Ueda, K Nishida, R Namikawa, M Seto, T Maruyama, S Takamoto, H Matsuoka, S Torii, K Ota
• Journal: Clinical and experimental immunology (Clin Exp Immunol) Vol. 58 Issue 2 Pg. 444-52 (Nov 1984) ISSN: 0009-9104 [Print] England
• PMID: 6333946 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antigens

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Antigens (analysis)
• Autoradiography
• Cell Separation
• Cells, Cultured
• Flow Cytometry
• Hematopoietic Stem Cells (immunology)
• Humans
• Leukemia (immunology)
• Lymphoma (immunology)
• Mice
• Mice, Inbred BALB C
• Rosette Formation
• T-Lymphocytes (immunology)