HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity is present in Ehrlich ascites tumor cells.

Abstract
The presence of a soluble, Mg2+- or Mn2+-dependent p-nitrophenylphosphatase activity in Ehrlich ascites tumor cell homogenates is reported. The crude homogenate was fractionated over Sephadex G-150 gel-filtration and DEAE-Sephacel anion-exchange columns, and two p-nitrophenylphosphatase activities were resolved. The most active fraction, Peak I, was characterized and found to be similar to phosphotyrosyl-protein phosphatases characterized elsewhere in that it has optimal activity at neutral pH; it is inhibited by phosphate, Zn2+, and vanadate; and it is not inhibited by levamisole. However, Peak I differs from phosphotyrosyl-protein phosphatases in that Mg2+ or Mn2+ is required for activity, fluoride is an inhibitor, and pyrophosphate is not inhibitory. Inhibition by the phosphorylated compounds phosphotyrosine, phosphoserine, phosphothreonine, ATP, CTP, GTP, ITP, NADP, fructose 6-phosphate, glucose 1-phosphate, galactose 1-phosphate, 2-phosphogluconic acid, and 6-phosphogluconic acid was also observed. Ehrlich ascites tumor cell p-nitrophenylphosphatase is shown to be sensitive to inactivation by trypsin, N-ethylmaleimide, or heat treatments.
AuthorsI Javeri, J Maxwell, O M Howard, S Yunker, S G O'Neal
JournalArchives of biochemistry and biophysics (Arch Biochem Biophys) Vol. 232 Issue 1 Pg. 214-22 (Jul 1984) ISSN: 0003-9861 [Print] United States
PMID6331318 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Manganese
  • Phosphoric Monoester Hydrolases
  • 4-Nitrophenylphosphatase
  • Trypsin
  • Magnesium
  • Ethylmaleimide
Topics
  • 4-Nitrophenylphosphatase (antagonists & inhibitors, metabolism)
  • Animals
  • Carcinoma, Ehrlich Tumor (enzymology)
  • Chromatography, Gel
  • Ethylmaleimide (pharmacology)
  • Hot Temperature
  • Hydrogen-Ion Concentration
  • Magnesium (metabolism)
  • Male
  • Manganese (metabolism)
  • Mice
  • Mice, Inbred Strains
  • Phosphoric Monoester Hydrolases (metabolism)
  • Phosphorylation
  • Substrate Specificity
  • Trypsin (pharmacology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: