The effects of cyclic AMP and cyclic GMP on redox state and energy state in hypoxic rat atria.

Abstract

The effects of 8-bromo-cAMP (10(-4)M) and 8-bromo-cGMP (10(-4)M) on tissue lactate, NADH, creatine phosphate (CP), ATP, ADP and AMP were studied in hypoxic (50% oxygen saturation) spontaneously beating rat atria. CP/ATP ratio and energy charge (EC) were also calculated. In hypoxic rat atria there was a significant increase in tissue lactate, NADH, ADP and AMP and a decrease in CP, CP/ATP ratio and EC. 8-bromo-cGMP abolished these hypoxia-induced changes. The levels of lactate and NADH decreased and those of CP, ATP, ADP and EC increased after administration of 8-bromo-cGMP. 8-bromo-cAMP increased the level of lactate during hypoxia, but did not affect the other hypoxia-induced changes. The present work indicates that cyclic GMP can change the redox state and energy state in a more favourable direction for the hypoxic rat heart.

• Authors: P Vuorinen, K Laustiola, T Metsä-Ketelä
• Journal: Life sciences (Life Sci) Vol. 35 Issue 2 Pg. 155-61 (Jul 09 1984) ISSN: 0024-3205 [Print] Netherlands
• PMID: 6330484 (Publication Type: Journal Article)

Chemical References

• Adenine Nucleotides
• Lactates
• Phosphocreatine
• NAD
• 8-Bromo Cyclic Adenosine Monophosphate
• 8-bromocyclic GMP
• Lactic Acid
• Cyclic GMP

Topics

• 8-Bromo Cyclic Adenosine Monophosphate (pharmacology)
• Adenine Nucleotides (metabolism)
• Animals
• Cyclic GMP (analogs & derivatives, pharmacology)
• Energy Metabolism (drug effects)
• Heart Atria (metabolism)
• Hypoxia (metabolism)
• Lactates (metabolism)
• Lactic Acid
• Male
• Myocardium (metabolism)
• NAD (metabolism)
• Oxidation-Reduction
• Phosphocreatine (metabolism)
• Rats
• Rats, Inbred Strains