Simplified single radial immunodiffusion detection of albumin receptors on HBsAG particles and its significance regarding sero-changes of HBeAG in hepatitis-B patients.
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| Abstract |
A simplified single radial immunodiffusion (SRID) method to detect HBsAg particles albumin receptor activity in regard to polymerized human serum albumin (pHSA) (binding-activity of pHSA = pHSA-BA or albumin receptor activity) was developed and its significance in HBeAg-positive hepatitis B patients after a one year follow-up study was discussed. The pHSA-BA determined by the simplified SRID method was classified into 5 grades; +, +/- and -, and the results of this grading of test sera agreed well with those obtained by the ELISA quantitative assay. According to the results of one year follow-up study in sero-changes of HBeAg in 35 HBeAg-positive cases, 7 cases with pHSA-BA did not show HBeAg sero-change, but of 16 cases with pHSA-BA and 12 cases with (+) pHSA-BA 3 (18.8%) and 7 (58.3%) cases showed HBeAg sero-changes, respectively. These results suggest that the present simplified SRID detection of albumin receptor on HBsAg particles might be a useful method for the laboratory investigation of hepatitis B patients.
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| Authors | T Tsuji, K Takahashi, K Naito, H Nagashima |
| Journal | Gastroenterologia Japonica (Gastroenterol Jpn) Vol. 19 Issue 2 Pg. 116-20 (Apr 1984) ISSN: 0435-1339 [Print] Japan |
| PMID | 6329888 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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