Effect of N-phenyl-N'-3-hydroxyphenylthiourea (PTU-23) on the protein synthesis in Krebs-II cells infected with encephalomyocarditis virus.

Abstract

PTU-23, a selective inhibitor of the reproduction of encephalomyocarditis (EMC) virus in Krebs-II ascites carcinoma cells, counteracts the virus-induced shut-off of the host-cell protein synthesis reducing it from 32% to 19%. The compound does not inhibit the synthesis of virus-specific proteins; the electrophoretic profile in SDS-PAAG shows an increase in the peaks of some viral polypeptides, mainly A, E and epsilon. It is suggested that this effect is connected to the partial inhibition by PTU-23 of the synthesis of the viral 37S RNA, without affecting its translation activity and also to the inhibition of the synthesis of 20S (RF) RNA--an inhibitor of the virus-specific protein synthesis. PTU-23 does not affect the processing of the high-molecular-weight viral precursor polypeptide preA, which is carried out by the virus-specific protease protein p22, as shown in studies with a cell-free system.

• Authors: A S Galabov, V A Ginevskaya, Svitkin YuV
• Journal: Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie = International journal of microbiology and hygiene. A, Medical microbiology, infectious... (Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol) Vol. 254 Issue 3 Pg. 306-17 (May 1983) ISSN: 0174-3031 [Print] Germany
• PMID: 6326422 (Publication Type: Journal Article)

Chemical References

• Antiviral Agents
• Neoplasm Proteins
• Viral Proteins
• N-phenyl-N'-3-hydroxyphenylthiourea
• Phenylthiourea

Topics

• Animals
• Antiviral Agents (pharmacology)
• Carcinoma, Krebs 2 (metabolism, microbiology)
• Cells, Cultured
• Electrophoresis, Polyacrylamide Gel
• Encephalomyocarditis virus (growth & development, metabolism)
• Mice
• Neoplasm Proteins (biosynthesis)
• Phenylthiourea (analogs & derivatives, pharmacology)
• Viral Proteins (biosynthesis)