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Kinetic effects of terbium ions on muscarinic acetylcholine receptors of murine neuroblastoma cells.

Abstract
Preincubation of murine neuroblastoma cells (clone N1E-115) with terbium chloride resulted in a significant potentiation of carbachol-mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6-fold decrease in carbachol concentration producing half-maximal responses, in addition to a significant increase in the Hill coefficient. Terbium ions also caused a significant decrease in the affinity and an increase in the maximum binding of [3H]quinuclidinyl benzilate to muscarinic receptors, the change in affinity being mainly due to a decrease in the association rate. Preincubation of cells with 1 mM carbachol for 4 h (the desensitized state of the muscarinic receptor) resulted in a decrease in the ability of terbium to alter [3H]quinuclidinyl benzilate binding. The effects of terbium reported here might be due to its affecting muscarinic receptor-effector coupling, which is considered to be lost upon receptor desensitization.
AuthorsE E El-Fakahany, M Pfenning, E Richelson
JournalJournal of neurochemistry (J Neurochem) Vol. 42 Issue 3 Pg. 863-9 (Mar 1984) ISSN: 0022-3042 [Print] England
PMID6319610 (Publication Type: Journal Article)
Chemical References
  • Receptors, Muscarinic
  • Terbium
  • Quinuclidinyl Benzilate
  • Cyclic GMP
Topics
  • Animals
  • Cell Line
  • Cyclic GMP (metabolism)
  • Kinetics
  • Mice
  • Neuroblastoma (metabolism)
  • Quinuclidinyl Benzilate (metabolism)
  • Receptors, Muscarinic (drug effects, metabolism)
  • Terbium (pharmacology)

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