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Characterization of alpha 2-adrenergic receptors in the human colon adenocarcinoma cell line HT 29 in culture by [3H]yohimbine binding.

Abstract
The presence of specific binding sites for [3H]yohimbine, a labelled alpha 2-adrenergic agent, on crude membranes of HT 29 cells established in culture from a human colon adenocarcinoma, is reported. The estimated affinity and number of sites (KD = 6.3 +/- 0.9 nM; Bmax = 224 +/- 28 fmol/mg protein) as well as the relative potencies of adrenergic agonists (clonidine greater than phenylephrine greater than amidephrine) and adrenergic antagonists (yohimbine greater than dihydroergotamine much greater than prazosin) to displace [3H]yohimbine binding indicate that the yohimbine sites of these cancer cells have similar characteristics to the alpha 2-adrenergic receptors described in other tissues.
AuthorsC Carpene, H Paris, C Cortinovis, V Viallard, J C Murat
JournalGeneral pharmacology (Gen Pharmacol) Vol. 14 Issue 6 Pg. 701-3 ( 1983) ISSN: 0306-3623 [Print] England
PMID6319226 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Neoplasm Proteins
  • Receptors, Adrenergic, alpha
  • Yohimbine
Topics
  • Adenocarcinoma (metabolism)
  • Cell Line
  • Cell Membrane (metabolism)
  • Colonic Neoplasms (metabolism)
  • Humans
  • Kinetics
  • Neoplasm Proteins (metabolism)
  • Receptors, Adrenergic, alpha (classification, metabolism)
  • Yohimbine (metabolism)

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