Products derived from 5,8,11-eicosatrienoic acid by the 5-lipoxygenase-leukotriene pathway.

Abstract

Analysis of products derived from 5,8,11-eicosatrienoic acid via the 5-lipoxygenase-leukotriene pathway showed that this fatty acid is readily converted to leukotriene (LT)A3. When 10,000 X g supernatant from rat basophilic leukemia cell homogenates was incubated with 30 microM fatty acid, 5,8,11-eicosatrienoic acid produced 6.2 +/- 1.1 nmol of LTA3 and arachidonic acid 15.5 +/- 1.9 nmol of LTA4 (n = 4). However, only insignificant amounts of LTB3 were formed (0.15 +/- 0.04 nmol of LTB3 and 4.2 +/- 0.4 nmol of LTB4, n = 4). These data indicate that the LTA-hydrolase requires not only the three double bonds of the triene but also the double bond at C-14 to efficiently convert LTA to LTB. These findings have significant implications for essential fatty acid deficiency.

• Authors: B A Jakschik, A R Morrison, H Sprecher
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 258 Issue 21 Pg. 12797-800 (Nov 10 1983) ISSN: 0021-9258 [Print] United States
• PMID: 6313677 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Arachidonic Acids
• Fatty Acids, Unsaturated
• Leukotriene A4
• Arachidonate Lipoxygenases
• Lipoxygenase
• 8,11,14-Eicosatrienoic Acid
• mead acid

Topics

• 8,11,14-Eicosatrienoic Acid (metabolism)
• Animals
• Arachidonate Lipoxygenases
• Arachidonic Acids (biosynthesis, isolation & purification)
• Basophils (enzymology)
• Cell Line
• Chromatography, High Pressure Liquid
• Fatty Acids, Unsaturated (metabolism)
• Isomerism
• Leukemia, Experimental (enzymology)
• Leukotriene A4
• Lipoxygenase (metabolism)
• Mass Spectrometry
• Rats