Abstract |
Various microbial products are known to influence the function of mouse peritoneal macrophages. Lipopolysaccharide (LPS) and certain lipid A-associated proteins are known to enhance the tumoricidal effects of macrophages. The purpose of this study was to determine whether porins (outer membrane proteins) of Salmonella typhimurium G30/ C21 would influence the activity of macrophages from lipid A-responsive and -unresponsive mice. Porins, extracted by a combined sodium dodecyl sulfate- EDTA method from cell walls, were free of LPS as determined by Limulus amebocyte lysate assay and appeared as a band at approximately 36,000 molecular weight on sodium dodecyl sulfate- polyacrylamide gel electrophoresis. In tumor cell killing assays done under LPS-free conditions, the porins in doses of 1 to 10 ng/ml enhanced the tumoricidal effect of macrophages from bacillus Calmette-Guérin-infected C3H/HeN or C3H/HeJ mice. Protein-free LPS enhanced the tumoricidal activity of macrophages from bacillus Calmette-Guérin-infected C3H/HeN but not C3H/HeJ mice. The tumoricidal-enhancing activity of protein-free LPS was blocked by the lipid A-binding antibiotic polymyxin B sulfate, but the effects of porins were not altered by the polymyxin B sulfate. These results suggest that porins, proteins known to alter membrane function, may alter macrophage function by interaction with macrophage membranes.
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Authors | J B Weinberg, E Ribi, R W Wheat |
Journal | Infection and immunity
(Infect Immun)
Vol. 42
Issue 1
Pg. 219-23
(Oct 1983)
ISSN: 0019-9567 [Print] United States |
PMID | 6311745
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- BCG Vaccine
- Bacterial Proteins
- Lipopolysaccharides
- Membrane Proteins
- Porins
- Polymyxin B
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Topics |
- Animals
- BCG Vaccine
(immunology)
- Bacterial Proteins
(pharmacology)
- Cell Line
- Cytotoxicity, Immunologic
(drug effects)
- Female
- Lipopolysaccharides
(pharmacology)
- Macrophages
(immunology)
- Membrane Proteins
(pharmacology)
- Mice
- Mice, Inbred C3H
- Polymyxin B
(pharmacology)
- Porins
- Salmonella typhimurium
(analysis)
- Sarcoma, Experimental
(immunology)
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