Effect of clofibrate feeding on palmitate and branched-chain 2-oxo acid oxidation in rat liver and muscle.
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| Abstract |
Oxidation rates of palmitate (total and antimycin-insensitive), pyruvate, leucine, 4-methyl-2-oxopentanoate and 3-methyl-2-oxobutanoate and activities of two mitochondrial marker enzymes (citrate synthase and cytochrome c oxidase) were assayed in liver and muscle homogenates of fed, clofibrate-treated and 18 hr-starved rats. Significant alterations in the clofibrate-treated and the starved rats were predominantly observed in the liver. Clofibrate feeding increased antimycin-insensitive (peroxisomal) and antimycin-sensitive (mitochondrial) palmitate oxidation and 4-methyl-2-oxopentanoate and pyruvate oxidation in liver. In muscle, only the activities of citrate synthase and cytochrome c oxidase were slightly decreased. Short starvation increased antimycin-sensitive palmitate and 4-methyl-2-oxopentanoate oxidation in liver. The rates of pyruvate and 3-methyl-2-oxobutanoate oxidation were decreased in muscle homogenates. Results suggest that myopathic phenomena observed after chronic clofibrate administration are not related to changes in the capacity of oxidative metabolism of muscle.
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| Authors | J F Glatz, A J Wagenmakers, J H Veerkamp, H T van Moerkerk |
| Journal | Biochemical pharmacology (Biochem Pharmacol) Vol. 32 Issue 17 Pg. 2489-93 (Sep 01 1983) ISSN: 0006-2952 [Print] England |
| PMID | 6311221 (Publication Type: Journal Article) |
| Chemical References |
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