Purification and properties of an altered form of elongation factor 2 from mutant cells resistant to intoxication by diphtheria toxin.

Abstract

Elongation factor 2 (EF-2) was isolated from wild-type and mutant-polyoma-virus-transformed baby hamster kidney cells resistant to intoxication by diphtheria toxin. Cells were grown as tumors in hamsters and EF-2 was purified from tissue homogenates by column chromatography. Both forms of EF-2 chromatograph identically in Whatman DE-52 DEAE-cellulose, Sephadex DEAE-A50 and Sephacryl S-200 resins. However, wild-type and the mutant form of EF-2 elute from phosphocellulose at 0.16 M and 0.24 M KCl respectively. Both forms of EF-2 migrate in sodium dodecyl sulfate/polyacrylamide gels as a single band with an Mr of 93000 and produce identical 125I-labeled tryptic peptide maps. However, additional labeled tryptic peptides are seen when wild-type EF-2 is ADP-ribosylated by fragment A diphtheria toxin. The purified mutant protein is totally resistant to ADP-ribosylation and cannot be transformed into an ADP-ribosylatable form in a posttranslational modification system in vitro, indicating that resistance to ADP-ribosylation results from a mutation in the structural gene for EF-2.

• Authors: W J Iglewski, H Lee
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 134 Issue 2 Pg. 237-40 (Aug 01 1983) ISSN: 0014-2956 [Print] England
• PMID: 6307688 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Diphtheria Toxin
• Peptide Elongation Factor 2
• Peptide Elongation Factors
• Adenosine Diphosphate Ribose

Topics

• Adenosine Diphosphate Ribose (metabolism)
• Animals
• Cell Line
• Cell Transformation, Viral
• Cricetinae
• Diphtheria Toxin (toxicity)
• Drug Resistance
• Kidney (analysis)
• Mutation
• Peptide Elongation Factor 2
• Peptide Elongation Factors (isolation & purification, metabolism)
• Polyomavirus