Abstract |
Human papovavirus JC, previously passaged in amnion cells, produced a cytopathic effect in urine-derived epithelial cells, and virus-specific antigens were demonstrable by indirect immunofluorescence. The hemagglutinating titer of JCV purified from infected cell cultures was generally 100- to 1000-fold higher than the amount of viral hemagglutinin used to initiate infection. Amnion-passaged JCV was readily adapted to growth in urine-derived epithelial cells. The prototype strain of human papovavirus BK, adapted to growth in human embryonic lung cells, also productively infected urine-derived epithelial cells. Primary human fetal glial cells and urine-derived cells were used in parallel experiments for primary isolation of JCV from diseased brain tissue. For this purpose, primary human fetal glial cells were more sensitive than urine-derived epithelial cells. Primary isolations of JCV and BKV from urine sediments of transplant patients and a normal male were made in urine-derived cells. Two renal transplant patients were identified as simultaneously excreting JCV and BKV. Both JCV and BKV genomes were molecularly cloned from one urine specimen of a double excretor. Although direct comparisons between primary human fetal glial and urine-derived epithelial cells were not made, it appears that the latter may be more suitable for primary isolation of JCV from urine.
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Authors | A M Beckmann, K V Shah |
Journal | Progress in clinical and biological research
(Prog Clin Biol Res)
Vol. 105
Pg. 3-14
( 1983)
ISSN: 0361-7742 [Print] United States |
PMID | 6304767
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Topics |
- BK Virus
(growth & development, isolation & purification)
- Cells, Cultured
- Epithelium
- Hemagglutination Inhibition Tests
- Hemagglutination Tests
- Humans
- In Vitro Techniques
- JC Virus
(growth & development, isolation & purification)
- Male
- Polyomavirus
(growth & development)
- Virus Cultivation
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