Inhibition of herpes simplex virus DNA synthesis by pokeweed antiviral protein.

Abstract

Pokeweed antiviral protein at a concentration of 3 microM inhibited both the synthesis and release of infectious herpes simplex virus type 1 in cell culture by 90 and 99%, respectively. Addition of pokeweed antiviral protein to Vero cell monolayers before virus infection was 10 to 15% more effective in reducing virus yields than was the simultaneous addition of the antiviral protein with virus inoculum. Viral DNA synthesis was inhibited by 90% in cells which had been exposed to the antiviral protein, whereas cellular DNA synthesis was unaffected. No significant inhibition in the synthesis of the majority of viral infected-cell polypeptides was observed early postinfection (7 h), with the exception of infected cell polypeptides 4 and 41, whose syntheses were reduced by 38 and 25%, respectively. At 9 to 21 h postinfection, however, the synthesis of individual infected cell polypeptides was reduced by 48 to greater than 99%.

• Authors: G J Teltow, J D Irvin, G M Aron
• Journal: Antimicrobial agents and chemotherapy (Antimicrob Agents Chemother) Vol. 23 Issue 3 Pg. 390-6 (Mar 1983) ISSN: 0066-4804 [Print] United States
• PMID: 6303210 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• DNA, Viral
• Plant Proteins
• Ribosome Inactivating Proteins, Type 1
• Viral Proteins
• N-Glycosyl Hydrolases
• pokeweed antiviral protein

Topics

• Animals
• Antiviral Agents (pharmacology)
• Cells, Cultured
• DNA, Viral (biosynthesis)
• Haplorhini
• Kinetics
• N-Glycosyl Hydrolases
• Plant Proteins (pharmacology)
• Ribosome Inactivating Proteins, Type 1
• Simplexvirus (drug effects, metabolism)
• Viral Proteins (biosynthesis)