| Abstract | Three genetic loci affecting tumor morphology lie within pTiA6NC T-DNA: tms, tmr, and tml. Using deletions and multiple transposon insertions, we constructed tumor-inducing (Ti) plasmids representing every possible double and triple mutant combination. tms tmr and tms tmr tml mutants did not incite tumors on most plants and produced a very weak response on a few other hosts but tms tml and tmr tml mutants were virulent. Thus, either tms+ or tmr+ alone can promote significant tumor growth but tml+ by itself is not sufficient. On hosts where tms mutants induce tumors accompanied by shoot proliferation, addition of a tml mutation reduces or eliminates shoot proliferation, suggesting that tml+ promotes shoot development. The small calli incited by tms tmr and tms tmr tml mutants contain agropine, an indication that these plant cells incorporate T-DNA in the absence of substantial tumor growth. |
| Authors | L W Ream, M P Gordon, E W Nester |
| Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 80
Issue 6
Pg. 1660-4
(Mar 1983)
ISSN: 0027-8424 UNITED STATES |
| PMID | 6300864
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- DNA Transposable Elements
- Plant Growth Regulators
|
| Topics |
- DNA Transposable Elements
- Mutation
- Plant Growth Regulators
(physiology)
- Plant Tumors
(genetics)
- Plasmids
- Rhizobium
(genetics)
|
