Abstract |
The distribution and numbers of human natural killer (NK) cells in different lymphoid tissues and several tumors were determined with the use of monoclonal antibody to the Leu-7 antigen and immunohistologic methods. Anti-Leu-7 reacts with large granular lymphocytes containing most of NK activity in the peripheral blood. The Leu-7+ cells were found mainly in the germinal centers of secondary follicles in lymph nodes, spleens, and tonsils. The mean number of Leu-7+ cells in 40 germinal centers was 223 +/- 103 SD. Only rare cells were found in the thymus. In the follicles studied in serial sections, the distribution of the Leu-7+ cells was distinctly different from that of T cells, B cells and Ia+ cells. The Leu-7+ cells did not react with OKT 6 antibody specific for immature (control) thymocytes and Langerhans' cells. By double staining techniques, two populations of Leu-7+ cells were identified in lymphoid tissues: those that did and did not express the Ia-like antigen. The numbers and localization of the Leu-7+ cells varied in different tumors, but often the Leu-7+ cells surrounded the neoplastic nodules or were seen in contact with individual cancer cells. Anti-Leu-7 may be a useful reagent for monitoring the presence and localization of NK cells in normal and malignant human tissues.
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Authors | L Si, T L Whiteside |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 130
Issue 5
Pg. 2149-55
(May 1983)
ISSN: 0022-1767 [Print] United States |
PMID | 6300238
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Isoantigens
|
Topics |
- Adult
- Antibodies, Monoclonal
(immunology)
- Carcinoma, Hepatocellular
(immunology)
- Child
- Hodgkin Disease
(immunology)
- Humans
- Isoantigens
(analysis)
- Killer Cells, Natural
(immunology)
- Liver Neoplasms
- Lymph Nodes
(immunology)
- Lymphoid Tissue
(immunology)
- Lymphoma
(immunology, secondary)
- Palatine Tonsil
(immunology)
- Skin
(immunology)
- Thymus Gland
(immunology)
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