Mutagenic virus replication in human tumor cells.

Abstract

Herpes simplex virus was grown in different lines of human tumor and normal cells. The progeny virus was assayed for resistance to iododeoxycytidine, an indicator of a forward mutation in the virus genome. Virus grown in cells from 4 of 5 tumor lines demonstrated greater fractions mutated to iododeoxycytidine resistance than did virus grown in 7 normal human skin cell lines. The data indicate that some lines of human tumor cells modify the herpesvirus replication process, making it more mutagenic. In 2 cases of osteosarcoma patients, normal skin fibroblasts of the patients yielded normal levels of mutagenesis, while their tumor cells gave enhanced mutagenesis.

• Authors: L E Bockstahler, C D Lytle, A S Lubiniecki, J M Cantwell, J A Galleshaw
• Journal: Cancer letters (Cancer Lett) 1982 Nov-Dec Vol. 17 Issue 2 Pg. 141-5 ISSN: 0304-3835 [Print] Ireland
• PMID: 6299510 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Deoxycytidine
• Bromodeoxycytidine
• ibacitabine

Topics

• Animals
• Bromodeoxycytidine (analogs & derivatives)
• Cell Line
• Chlorocebus aethiops
• Deoxycytidine (analogs & derivatives, pharmacology)
• Drug Resistance, Microbial
• Glioma
• Humans
• Kidney
• Lung Neoplasms
• Melanoma
• Mutation
• Osteosarcoma
• Simplexvirus (drug effects, genetics)
• Skin
• Virus Replication