Human renal carcinoma cells produce hypercalcemia in the nude mouse and a novel protein recognized by parathyroid hormone receptors.

Abstract

When grown in nude mice, cultured renal carcinoma cells from a hypercalcemic patient produced marked hypercalcemia that was reversed by resection of tumor. Conditioned medium from this cell line contained a protein with activity in a renal adenylate cyclase bioassay for parathyroid hormone (PTH) which was blocked by the competitive PTH antagonist [8norleucyl, 18norleucyl, 34tyrosinyl]bPTH (3-34)amide. However, the biologically active protein was eluted from gel filtration columns as a larger molecular size component that PTH and was not recognized by any of four region-specific PTH antisera. The properties of this factor resemble those of the postulated PTH-like substance(s) in humoral hypercalcemia of malignancy.

• Authors: G J Strewler, R D Williams, R A Nissenson
• Journal: The Journal of clinical investigation (J Clin Invest) Vol. 71 Issue 3 Pg. 769-74 (Mar 1983) ISSN: 0021-9738 [Print] United States
• PMID: 6298282 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Neoplasm Proteins
• Receptors, Cell Surface
• Receptors, Parathyroid Hormone

Topics

• Adenocarcinoma (complications, metabolism)
• Animals
• Humans
• Hypercalcemia (etiology)
• Kidney Neoplasms (complications, metabolism)
• Male
• Mice
• Mice, Nude
• Middle Aged
• Neoplasm Proteins (physiology)
• Neoplasm Transplantation
• Neoplasms, Experimental (complications)
• Receptors, Cell Surface (metabolism)
• Receptors, Parathyroid Hormone