Identification of a human neuroectodermal tumor antigen (OFA-I-2) as ganglioside GD2.

Abstract

Two monospecific human antibodies (anti-OFA-I-1 and anti-OFA-I-2) produced in vitro by lymphoblast cell lines originating from melanoma patients have been shown previously to recognize cell surface antigens (OFA-I-1 and OFA-I-2) on human tumors and fetal brain: OFA-I-1 is expressed on a variety of human tumors, while OFA-I-2 has been detected only on tumors of neuroectodermal origin. Evidence presented in this report suggests that the two antigens expressed by a cultured human melanoma cell line (M14) are chemically distinct and that OFA-I-2 is a cell surface glycolipid, ganglioside GD2: GalNAc beta 1 leads to 4 NeuAc alpha 2 leads to 8NeuAc alpha 2 leads to 3 Gal beta 1 leads to 4Glc-ceramide.

• Authors: L D Cahan, R F Irie, R Singh, A Cassidenti, J C Paulson
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 79 Issue 24 Pg. 7629-33 (Dec 1982) ISSN: 0027-8424 [Print] United States
• PMID: 6296843 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm
• Gangliosides
• Sialic Acids

Topics

• Antibodies, Monoclonal
• Antibody Specificity
• Antigens, Neoplasm (immunology)
• Ectoderm (immunology)
• Gangliosides (immunology)
• Humans
• Neoplasms, Nerve Tissue (immunology)
• Sialic Acids (analysis)