Improved immunofluorescence antigens for detection of immunoglobulin M antibodies to Epstein-Barr viral capsid antigen and antibodies to Epstein-Barr virus nuclear antigen.

Abstract

Epstein-Barr viral capsid antigen and nuclear antigen produced by modified procedures were evaluated for use in measuring viral capsid antigen immunoglobulin M and Epstein-Barr virus nuclear antigen antibody responses in sera from patients with suspected Epstein-Barr virus infections. Viral capsid antigen production was stimulated with a phorbol ester, and the Epstein-Barr virus nuclear antigen cells were fixed in suspension to eliminate loss of antigen during the drying process. Both preparations proved to be sensitive and reliable.

• Authors: D Gallo, K H Walen, J L Riggs
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 15 Issue 2 Pg. 243-8 (Feb 1982) ISSN: 0095-1137 [Print] United States
• PMID: 6279691 (Publication Type: Journal Article)

Chemical References

• Antibodies, Viral
• Antigens, Viral
• Immunoglobulin M
• Viral Proteins

Topics

• Adolescent
• Adult
• Animals
• Antibodies, Viral (analysis)
• Antigens, Viral (immunology)
• Capsid (immunology)
• Cell Nucleus (immunology)
• Child
• Child, Preschool
• Fluorescent Antibody Technique
• Herpesvirus 4, Human (immunology)
• Humans
• Immunoglobulin M (analysis)
• Infant
• Middle Aged
• Tumor Virus Infections (immunology)
• Viral Proteins (immunology)