Formation of leukotrienes E3, E4 and E5 in rat basophilic leukemia cells.

Abstract

Rat basophilic leukemia (RBL-1) cells incubated with ionophore A23187 and 5,8,11-eicosatrienoic acid produced three slow-reacting substances identified as leukotrienes C3, D3 and E3 by spectroscopic, chromatographic and enzymatic methods. 5,8,11,14,17-Eicosapentaenoic acid was similarly converted by RBL-1 cells to leukotrienes C5, D5. and E5. Leukotrienes C4, D4 and E4 were also formed in these experiments from endogenous arachidonic acid. Time-course studies, incubations with 3H-labeled leukotriene C3 and effects of acivicin [L-(alpha S, 5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid; a gamma-glutamyl transpeptidase inhibitor] indicated that leukotrienes C and D are intermediates in the formation of leukotrienes E. L-Cysteine enhanced the conversion of leukotriene C3 to leukotriene D3 and inhibited further degradation of leukotriene D3 to leukotriene E3.

• Authors: L Orning, K Bernström, S Hammarström
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 120 Issue 1 Pg. 41-5 (Nov 1981) ISSN: 0014-2956 [Print] England
• PMID: 6273168 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Arachidonic Acids
• Isoxazoles
• SRS-A
• Arachidonic Acid
• Calcimycin
• Leukotriene E4
• 8,11,14-Eicosatrienoic Acid
• mead acid
• Cysteine
• acivicin
• Glycine

Topics

• 8,11,14-Eicosatrienoic Acid (pharmacology)
• Animals
• Arachidonic Acid
• Arachidonic Acids (blood, metabolism)
• Basophils (metabolism)
• Calcimycin (pharmacology)
• Chromatography, High Pressure Liquid
• Cysteine (pharmacology)
• Glycine (analogs & derivatives, pharmacology)
• Isoxazoles (pharmacology)
• Leukemia, Experimental (metabolism)
• Leukotriene E4
• Rats
• SRS-A (blood)