Abstract |
Monoclonal antisera against mouse Thy 1.2 antigen were used to assess the expression of Thy 1.2 antigen on BALB/c and BALB/cfC3H normal mouse mammary gland epithelial cells (MMEC) and on spontaneous and transplanted BALB/cfC3H mammary adenocarcinoma cells. Immunofluorescence (IF) assays with the monoclonal anti-Thy 1.2 sera demonstrated that Thy 1.2 antigen was not expressed on the surface membranes of MMEC (both virus-free BALB/c and virus-infected BALB/cfC3H) and tumor (BALB/cfC3H) mammary epithelial cells. A specific antiserum against MMEC was used to establish the expression of this normal antigen(s) on the surfaces of both MMEC and tumor mammary epithelial cells obtained from virus-free and virus-infected mice. In addition, the expression of the mammary tumor virus envelope glycoprotein gp52 was determined by indirect IF on the surfaces of virus-infected mammary epithelial cells, both MMEC and tumor cells.
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Authors | K Thompson |
Journal | Journal of the National Cancer Institute
(J Natl Cancer Inst)
Vol. 67
Issue 1
Pg. 143-8
(Jul 1981)
ISSN: 0027-8874 [Print] United States |
PMID | 6265679
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies
- Antibodies, Monoclonal
- Isoantigens
- Viral Proteins
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Topics |
- Adenocarcinoma
(immunology)
- Animals
- Antibodies
- Antibodies, Monoclonal
- Cell Membrane
(immunology)
- Epithelium
(immunology)
- Female
- Isoantigens
(analysis)
- Mammary Glands, Animal
(immunology)
- Mammary Neoplasms, Experimental
(immunology)
- Mammary Tumor Virus, Mouse
(immunology)
- Mice
- Mice, Inbred BALB C
- T-Lymphocytes
(immunology)
- Viral Proteins
(analysis, immunology)
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