Role of antibody to leukocytosis-promoting factor hemagglutinin and to filamentous hemagglutinin in immunity to pertussis.

Abstract

Gamma globulins were obtained from rabbits hyperimmunized with the filamentous hemagglutinin (FHA) or the leukocytosis-promoting factor hemagglutinin (LPF.HA) of Bordetella pertussis. Double immunodiffusion analyses showed the antibodies to be highly specific for the homologous antigen. Intraperitoneal injection of anti-FHA or anti-LPF.HA into 10-day-old ICR mice 30 min before aerosol challenge with B. pertussis protected the mice from disease as evidenced by survival and a normal rate of gain in body weight. Both anti-LPF.HA and anti-FHA enhanced the clearance of B. pertussis from the lung and prevented leukocytosis which normally occurs after aerosol infection of mice with B. pertussis. Anti-LPF.HA, but not anti-FHA, neutralized the leukocytosis-promoting and histamine-sensitizing activities of LPF.HA when incubated with the purified protein in vitro. Anti-FHA, but not anti-LPF.HA, prevented the attachment of B. pertussis to mammalian cells (HeLa and Vero) in culture.

• Authors: Y Sato, K Izumiya, H Sato, J L Cowell, C R Manclark
• Journal: Infection and immunity (Infect Immun) Vol. 31 Issue 3 Pg. 1223-31 (Mar 1981) ISSN: 0019-9567 [Print] United States
• PMID: 6262248 (Publication Type: Journal Article)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Hemagglutinins
• Interleukin-2
• Lymphokines
• Pertussis Vaccine
• Virulence Factors, Bordetella
• Pertussis Toxin

Topics

• Animals
• Antibodies, Bacterial
• Antigens, Bacterial (analysis)
• Bordetella pertussis (immunology)
• Disease Models, Animal
• Hemagglutinins (immunology)
• Immunization, Passive
• Interleukin-2 (immunology)
• Lymphokines (immunology)
• Mice
• Pertussis Toxin
• Pertussis Vaccine (analysis)
• Rabbits
• Virulence Factors, Bordetella