Synthesis of catechol estrogens by human uterus and leiomyoma.

Abstract

Homogenates of human endometrial, myometrial and leiomyoma tissues were incubated with (2,4,6,7-3H)-estradiol and tritiated catechol estrogens were isolated and identified. Though 2- and 4-hydroxylations were about the same in endometrium, 4-hydroxylation was two to four fold higher than 2-hydroxylation in myometrium and leiomyoma. However, endometrium showed greater capacity to form both 2- and 4-hydroxyestrogens than the other two tissues. Both 2- and 4-hydroxylations were significantly less than in myometrium. In view of the reports indicating that inhibitors of catechol 0-methyl transferase (COMT) might act as antineoplastic agents due to their interference with t-RNA methylases and since catechol estrogens inhibit COMT, the present results suggest that endogenous synthesis of catechol estrogens may play an important role in the pathophysiology of uterine leiomyoma.

• Authors: V V Reddy, P Hanjani, R Rajan
• Journal: Steroids (Steroids) Vol. 37 Issue 2 Pg. 195-203 (Feb 1981) ISSN: 0039-128X [Print] United States
• PMID: 6261427 (Publication Type: Journal Article)

Chemical References

• Carbon Radioisotopes
• Catechols
• Estrogens
• Estrogens, Catechol
• Tritium
• Estradiol
• Steroid Hydroxylases
• Aryl Hydrocarbon Hydroxylases
• CYP1B1 protein, human
• Cytochrome P-450 CYP1A1
• Cytochrome P-450 CYP1B1
• estrogen 2-hydroxylase

Topics

• Aryl Hydrocarbon Hydroxylases
• Carbon Radioisotopes
• Catechols (biosynthesis)
• Cytochrome P-450 CYP1A1
• Cytochrome P-450 CYP1B1
• Endometrium (metabolism)
• Estradiol (metabolism)
• Estrogens (biosynthesis)
• Estrogens, Catechol
• Female
• Humans
• Leiomyoma (metabolism)
• Myometrium (metabolism)
• Steroid Hydroxylases (metabolism)
• Tritium
• Uterine Neoplasms (metabolism)
• Uterus (metabolism)