Abstract |
Levels of antirotaviral secretory immunoglobulin A were measured by enzyme-linked immunosorbent assay in colostrum and milk samples collected daily for the first 5 days postpartum from 49 mothers breast-feeding their infants. The trypsin-inhibitory capacity of these lacteal secretion samples was assessed by their ability to inhibit the hydrolysis of alpha-N-benzoyl-DL- arginine-p-nitroanilide by trypsin. Stools passed by these breast-fed infants and by an additional 43 bottle-fed infants were pooled by individual and examined by electron microscopy for rotavirus. Stool trypsin levels were estimated with the gelatin hydrolysis test. Breast-fed infants were significantly less likely to become infected with rotavirus and showed significantly lower stool tryptic activity than did bottle-fed infants. Breast-fed infants who did not excrete rotavirus over the 5-day period received milk of significantly higher antirotaviral secretory immunoglobulin A or trypsin-inhibitory capacity or both than breast-fed infants who were infected with rotavirus. A case of probable maternal rotavirus infection during pregnancy, producing greatly elevated lacteal antirotaviral secretory immunoglobulin A levels lasting for 2 years, was detected. Results of this study suggest that both antibodies and trypsin inhibitors in human milk can be associated with the protection of neonates against rotavirus infection in the first 5 days of life.
|
Authors | B S McLean, I H Holmes |
Journal | Journal of clinical microbiology
(J Clin Microbiol)
Vol. 13
Issue 1
Pg. 22-9
(Jan 1981)
ISSN: 0095-1137 [Print] United States |
PMID | 6257755
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Immunoglobulin A
- Immunoglobulin A, Secretory
- Trypsin Inhibitors
- Trypsin
|
Topics |
- Colostrum
(analysis)
- Feces
(enzymology)
- Humans
- Immunoglobulin A
(analysis)
- Immunoglobulin A, Secretory
(analysis)
- Infant, Newborn
- Infant, Newborn, Diseases
(immunology)
- Milk, Human
(analysis, immunology)
- Reoviridae
(immunology)
- Reoviridae Infections
(immunology)
- Rotavirus
(immunology)
- Trypsin
(metabolism)
- Trypsin Inhibitors
(analysis)
|