Persistence of genomes of both herpesvirus of turkeys and Marek's disease virus in a chicken T-lymphoblastoid cell line.

Abstract

A cell line tentatively designated as MDCC-BO1(T), was established from spleen cells of an apparently healthy chicken inoculated with herpesvirus of turkey (HVT). BO1(T) cells were T lymphoblastoid cells and the more than 95% of them had Marek's disease (MD) tumor-associated surface antigen (MATSA). However, no viral internal antigens or membrane antigens could be demonstrated in them by immunofluorescence tests using chicken anti-HVT and -MD virus (MDV) sera. The virus could be rescued from BO1(T) cells by co-cultivation with chick embryo fibroblasts (CEF). The DNA of the rescued virus was characterized as HVT DNA by its sedimentation profile in a neutral glycerol gradient and its endonuclease Hind III cleavage-pattern. Ultrastructural studies on CEF infected with the rescued virus revealed the presence of HVT-like virions. However, DNA-DNA reassociation kinetics showed that the BO1(T) cells contained a few copies of NVT and also MDV genomes.

• Authors: N Kitamoto, K Ikuta, S Kato, K Hirai
• Journal: Biken journal (Biken J) Vol. 23 Issue 1 Pg. 1-8 (Mar 1980) ISSN: 0006-2324 [Print] Japan
• PMID: 6254498 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral

Topics

• Animals
• Antigens, Viral (analysis)
• Cell Line
• Chickens
• Genes, Viral
• Herpesviridae (genetics, immunology, isolation & purification)
• Herpesvirus 2, Gallid (genetics, immunology)
• T-Lymphocytes (microbiology)
• Turkeys