Abstract |
1. Incubation of hepatocytes from fed or starved rats with increasing glucose concentrations caused a stimulation of lactate production, which was further increased under anaerobic conditions. 2. When glycolysis was stimulated by anoxia, [ fructose 2,6-bis- phosphate] was decreased, indicating that this ester could not be responsible for the onset of anaerobic glycolysis. In addition, the effect of glucose in increasing [ fructose 2,6-bisphosphate] under aerobic conditions was greatly impaired in anoxic hepatocytes. [ Fructose 2,6-bisphosphate] was also diminished in ischaemic liver, skeletal muscle and heart. 3. The following changes in metabolite concentration were observed in anaerobic hepatocytes: AMP, ADP, lactate and L- glycerol 3-phosphate were increased; ATP, citrate and pyruvate were decreased: phosphoenolpyruvate and hexose 6-phosphates were little affected. Concentrations of adenine nucleotides were, however, little changed by anoxia when hepatocytes from fed rats were incubated with 50 mM- glucose. 4. The activity of ATP: fructose 6-phosphate 2-phosphotransferase was not affected by anoxia but decreased by cyclic AMP. 5. The role of fructose 2,6-bisphosphate in the regulation of glycolysis is discussed.
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Authors | L Hue |
Journal | The Biochemical journal
(Biochem J)
Vol. 206
Issue 2
Pg. 359-65
(Aug 15 1982)
ISSN: 0264-6021 [Print] England |
PMID | 6216883
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Fructosediphosphates
- Hexosediphosphates
- Lactates
- Lactic Acid
- fructose 2,6-diphosphate
- Phosphofructokinase-1
- Glucose
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Topics |
- Anaerobiosis
- Animals
- Fructosediphosphates
(metabolism)
- Glucose
(pharmacology)
- Glycolysis
(drug effects)
- Hexosediphosphates
(metabolism)
- In Vitro Techniques
- Lactates
(metabolism)
- Lactic Acid
- Liver
(cytology, drug effects, metabolism)
- Phosphofructokinase-1
(metabolism)
- Rats
- Starvation
- Stimulation, Chemical
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