Transfer of a UV-damaged F sex factor to a recipient lambda lysogen induces prophage lambda development. Under these conditions
RecA protein synthesis was induced and
lambda repressor cleaved, as observed upon direct induction, that is, when the recipient lambda lysogen was directly exposed to UV-light. The efficiency of induction of
RecA protein synthesis in recipient bacteria which had received an irradiated F-lac factor was about 80% of that measured upon direct induction. We observed the simultaneous disappearance of
lambda repressor and a slight production of cleavage fragments; quantitation by densitometric scanning of the autoradiogram after correction for the efficiency of transfer indicated that 55% of
lambda repressor was cleaved. Transfer of UV-damaged Hfr
DNA failed to induce
RecA protein synthesis. A lambda phage vector carrying oriF, the cloned origin of F plasmid replication, after exposure to UV-light and
infection of a recipient lysogen, induced
RecA protein synthesis and a moderate but significant cleavage of
lambda repressor. Indirect induction by UV-damaged F sex factor or phage lambda oriF resulted in biochemical cellular reactions similar to those observed upon direct induction. LexA repressor that negatively controls
RecA protein synthesis appeared more susceptible to cleavage than did
lambda repressor.