Activated oxygen species produced by photoexcited furocoumarin derivatives.

Abstract

Furocoumarin derivatives (FCD) are investigated in order to determine their ability to photosensitize the production of activated oxygen species. Using the method based on the specific singlet oxygen (1O2) oxydation of cholesterol, all FCD except bergaten appeared to be able to produce 1O2 with various efficiencies. EPR spin trapping experiments show that photoexcited FCD produce hydroxyl radicals as detected by the formation of a DMPO-OH signal which can be abolished when the photosensitization reaction is carried out in the presence of specific OH scavengers. Moreover, the photo-ejection of hydrated electron (e-) by FCD is demonstrated by the loss of paramagnetic absorption of nitroxide free radicals as e- trap.

• Authors: J Decuyper, J Piette, A Van de Vorst
• Journal: Archives internationales de physiologie et de biochimie (Arch Int Physiol Biochim) Vol. 91 Issue 5 Pg. 471-6 (Dec 1983) ISSN: 0003-9799 [Print] Belgium
• PMID: 6204618 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Furocoumarins
• Hydroxides
• Hydroxyl Radical
• Cholesterol
• Oxygen

Topics

• Cholesterol (metabolism)
• Electron Spin Resonance Spectroscopy
• Furocoumarins (metabolism)
• Hydroxides (metabolism)
• Hydroxyl Radical
• Oxygen (metabolism)
• Photochemistry