Characterization of N-glycolyneuraminic acid-containing glycosphingolipids from a Marek's disease lymphoma-derived chicken cell line, MSB1, as tumor-associated heterophile Hanganutziu-Deicher antigens.

Abstract

Heterophile, Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acid-containing glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB1, by enzyme-immunoassay with chicken antibody against N-glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immunoassay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-beta-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGc alpha 2-2Gal beta 1-4Glc-Cer), NeuGc-nLcOse4Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer) and NeuGc-nLcOse6Cer (NeuGc alpha 2-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc-Cer).

• Authors: H Higashi, K Ikuta, S Ueda, S Kato, Y Hirabayashi, M Matsumoto, M Naiki
• Journal: Journal of biochemistry (J Biochem) Vol. 95 Issue 3 Pg. 785-94 (Mar 1984) ISSN: 0021-924X [Print] England
• PMID: 6202681 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Glycosphingolipids
• Neuraminic Acids
• N-glycolylneuraminic acid
• Glycoside Hydrolases
• keratan-sulfate endo-1,4-beta-galactosidase
• beta-Galactosidase

Topics

• Animals
• Antigens, Neoplasm (isolation & purification)
• Cell Line
• Chickens (immunology)
• Chromatography, Thin Layer
• Enzyme-Linked Immunosorbent Assay
• Glycoside Hydrolases
• Glycosphingolipids (isolation & purification)
• Immunoenzyme Techniques
• Marek Disease (immunology)
• Neuraminic Acids (isolation & purification)
• Staining and Labeling
• beta-Galactosidase