Fluctuations in phosphoribosyl pyrophosphate levels in monolayer tumor cell lines. Effects of drugs.

Abstract

The concentration of PRPP (phosphoribosyl pyrophosphate) measured in tumor cells grown in monolayer showed a large variation with the various harvesting methods examined, including trypsinization. This variation could be reduced by a 1-h incubation of trypsinized cells as a suspension in Dulbecco's medium. After this preincubation these cell suspensions were suitable for the study of modulation of PRPP. One microM methotrexate caused a 2-3-fold increase and 1 mM N- phosphonoacetyl -L-aspartate a slight increase, but inosine and deoxyinosine drastically reduced PRPP concentrations. 5-Fluorouracil had no effect. This study demonstrates that metabolic parameters such as PRPP concentrations can be studied conveniently in suspensions of cells which are commonly cultivated as monolayers.

• Authors: G J Peters, E Laurensse, A Leyva, H M Pinedo
• Journal: FEBS letters (FEBS Lett) Vol. 170 Issue 2 Pg. 277-80 (May 21 1984) ISSN: 0014-5793 [Print] England
• PMID: 6202553 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Buffers
• Pentosephosphates
• Purine Nucleosides
• Aspartic Acid
• Phosphoribosyl Pyrophosphate
• sparfosic acid
• Trypsin
• Phosphonoacetic Acid
• Methotrexate

Topics

• Animals
• Aspartic Acid (analogs & derivatives, pharmacology)
• Buffers
• Cell Line
• Cytological Techniques
• Fibroblasts (metabolism)
• Humans
• Melanoma (metabolism)
• Methotrexate (pharmacology)
• Mice
• Pentosephosphates (metabolism)
• Phosphonoacetic Acid (analogs & derivatives, pharmacology)
• Phosphoribosyl Pyrophosphate (metabolism)
• Purine Nucleosides (pharmacology)
• Trypsin (pharmacology)