Antiviral and protein-inducing activities of recombinant human leukocyte interferons and their hybrids.

Abstract

The antiviral activities of recombinant human leukocyte interferons IFN-alpha A and IFN-alpha D as well as five hybrids of these interferons against retroviruses, vesicular stomatitis virus, and encephalomyocarditis virus were studied in feline, human, and murine cells. Although these interferon species had widely different potencies, their activities against these viruses were, in general, proportional. The IFN-alpha A/D (Bgl) hybrid was the most potent species, and the IFN-alpha D/A (Bgl) hybrid was the least potent. However, the latter species did not interfere with the action of the former species. Like natural human leukocyte interferon, each of the seven species of recombinant interferons induced the synthesis of at least five proteins in human fibroblasts, whereas induction of only one such protein was readily detected in a feline fibroblast line in which these interferon species inhibited the replication of all three viruses.

• Authors: G C Sen, R Herz, V Davatelis, S Pestka
• Journal: Journal of virology (J Virol) Vol. 50 Issue 2 Pg. 445-50 (May 1984) ISSN: 0022-538X [Print] United States
• PMID: 6200607 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Interferon Type I
• Proteins
• RNA-Directed DNA Polymerase

Topics

• Animals
• Cats
• Cell Line
• Dose-Response Relationship, Drug
• Encephalomyocarditis virus (drug effects)
• Humans
• Interferon Type I (pharmacology)
• Mice
• Molecular Weight
• Protein Biosynthesis (drug effects)
• Proteins (isolation & purification)
• RNA-Directed DNA Polymerase (metabolism)
• Retroviridae (drug effects)
• Vesicular stomatitis Indiana virus (drug effects)