| Abstract | The ability of an azobenzenearsonate (ABA)-specific suppressor T cell factor, a soluble extract from first order suppressor T cells (Ts1), and suppressor molecules produced by a long-term T cell hybridoma to regulate ABA-specific granuloma formation was studied. ABA-derivatized syngeneic spleen cells (ABA-SC) administered subcutaneously induced persistent delayed-type hypersensitivity (DTH) responses, detected by footpad swelling and hapten-specific granuloma formation by 72 and 96 hr after challenge with ABA-bovine serum albumin coupled to polyacrylamide beads (ABA-BSA-PAB). Soluble factors from ABA-specific Ts1 prevented DTH and granulomatous development after subcutaneous administration of ABA-SC. Moreover, the in vivo administration of a factor that is derived from a Ts1 functioning hybrid cell line induced a second set of suppressor cells (Ts2) that upon transfer to syngeneic ABA-primed mice were able to inhibit granuloma formation in the footpad, as well as in the gastrointestinal tract after challenge with ABA-BSA-PAB. These experiments demonstrate the dependence of the granulomatous reaction on T cell-mediated events, as well as the potential therapeutic efficacy of an antigen-specific suppressor T cell factor and a hybridoma T cell product in limiting antigen-specific granuloma formation in vivo. |
| Authors | C H Ginsburg, J T Dambrauskas, R B Whitaker, Z M Falchuk, M I Greene |
| Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 132
Issue 1
Pg. 203-8
(Jan 1984)
ISSN: 0022-1767 UNITED STATES |
| PMID | 6197444
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
| Chemical References |
- Epitopes
- Lymphokines
- Suppressor Factors, Immunologic
- p-Azobenzenearsonate
|
| Topics |
- Animals
- Disease Models, Animal
- Epitopes
- Female
- Granuloma
(immunology, pathology, therapy)
- Hybridomas
(immunology, metabolism)
- Hypersensitivity, Delayed
(immunology, therapy)
- Lymphokines
(administration & dosage)
- Mice
- Mice, Inbred A
- Suppressor Factors, Immunologic
- T-Lymphocytes, Regulatory
(immunology, metabolism)
- p-Azobenzenearsonate
(immunology)
|
