Abstract |
In the 4-hydroxy-3-nitrophenyl acetyl (NP) contact sensitivity system, the activity of third-order suppressor cells and their factors is restricted by H-2(I-J) and Igh linked genes. The present report analyzes the specificity of NP-specific Ts3 cells and factors derived from H-2 and Igh heterozygous (B6 X C3H)F1 mice. Two approaches were used. First, heterogeneous populations of F1 Ts3 cells were activated in vitro and then assayed in Ts3-depleted recipients which carried different combinations of H-2 and Igh alleles. The second approach was to hybridize the Ts3 cells and analyze the specificity of the F1-derived TsF3. The combined data demonstrated four functionally distinct populations of Ts3 cells. The activity of each population was restricted by a particular combination of H-2 and Igh haplotypes. Thus, Ts3 cells derived from F1 donors can demonstrate an apparent scrambling of H-2 and Igh restriction specificities. There was functional allelic exclusion of the H-2(I-J) and Igh determinants expressed on (B6 X C3H)F1 hybridoma-derived TsF3. Thus, TsF3 from each cloned hybridoma line expressed only one set of I-J and Igh determinants. Furthermore, there was a complete correlation between the I-J and Igh linked determinants expressed on TsF3 and the restriction specificity. In view of the recent findings on the molecular biology of the I-J region, an alternative interpretation of the role of I-J determinants on suppressor cells and factors is offered.
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Authors | M Minami, I Aoki, N Honji, C R Waltenbaugh, M E Dorf |
Journal | The Journal of experimental medicine
(J Exp Med)
Vol. 158
Issue 5
Pg. 1428-43
(Nov 01 1983)
ISSN: 0022-1007 [Print] United States |
PMID | 6195283
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Epitopes
- H-2 Antigens
- Immunoglobulin Idiotypes
- Lymphokines
- Suppressor Factors, Immunologic
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Topics |
- Animals
- Epitopes
(genetics)
- Gene Expression Regulation
- H-2 Antigens
(genetics)
- Hybridomas
(immunology)
- Immunoglobulin Idiotypes
(immunology)
- Lymphocyte Activation
- Lymphokines
(genetics, immunology)
- Mice
- Mice, Inbred C3H
- Suppressor Factors, Immunologic
- T-Lymphocytes, Regulatory
(immunology)
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