Comparison of allogeneic and xenogeneic determinants on the H-2Kk molecule.

Abstract

Two monoclonal rat anti-H-2 antibodies were derived by cell fusion from a rat immunized with mouse CH-1 lymphoma cells. Both antibodies recognize polymorphic H-2 determinants expressed on most haplotypes. Competitive binding studies on H-2Kk show that one rat antibody, R1-9.6, detects a determinant that is distant from the allodeterminants defined by 12 different mouse monoclonal anti H-2 antibodies. In contrast, the rat antibody R1-21.2 inhibits the binding of most mouse monoclonal anti H-2 antibodies to H-2k cells, possibly by causing a conformational change in the H-2 molecule. Both antibodies bind strongly to H-2 molecules and can be used for structural and biochemical studies.

• Authors: S Koch, N Koch, P Robinson, G Hämmerling
• Journal: Transplantation (Transplantation) Vol. 36 Issue 2 Pg. 177-80 (Aug 1983) ISSN: 0041-1337 [Print] United States
• PMID: 6192569 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Epitopes
• Glycoproteins
• H-2 Antigens
• Isoantibodies

Topics

• Animals
• Antibodies, Monoclonal (immunology)
• Epitopes (immunology)
• Glycoproteins (immunology)
• H-2 Antigens (immunology)
• Isoantibodies (immunology)
• Mice
• Mice, Inbred Strains (immunology)
• Rats
• Rats, Inbred Lew (immunology)
• Species Specificity
• Spleen (immunology)