Isolation of early viral proteins from poxvirus-infected chick embryo fibroblasts by DNA-cellulose chromatography and inhibition of their synthesis by chicken interferon.

Abstract

Up to seven early poxvirus-specific proteins have been isolated from vaccinia-WR-infected and cowpox-virus-infected chick embryo fibroblasts by affinity chromatography on native DNA-cellulose columns. The proteins have been characterized by one-dimensional sodium dodecyl sulfate/polyacrylamide gel electrophoresis and by nonequilibrium pH-gradient electrophoresis. The molecular weights of the viral proteins were determined by comparison with proteins of known molecular weight and are comparable to several of the vaccinia-WR-specific DNA-binding proteins isolated previously from infected L-929 cells by Solosky J. M., Esteban M. and Holowczak J.A. [J. Virol. 25, 263-273 (1978)]. The viral proteins binding reversibly to native DNA have been classified as immediate early viral gene products. Synthesis of cowpox-virus-induced early DNA-binding proteins is inhibited in chick cells pretreated with homologous interferon at a concentration of 500--1000 units/ml.

• Authors: J Rösel, C Jungwirth
• Journal: European journal of biochemistry (Eur J Biochem) Vol. 132 Issue 2 Pg. 361-7 (May 02 1983) ISSN: 0014-2956 [Print] England
• PMID: 6188613 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA-Binding Proteins
• Viral Proteins
• Interferons
• DNA Helicases

Topics

• Animals
• Chick Embryo
• Chromatography, Affinity
• DNA Helicases (biosynthesis, isolation & purification)
• DNA-Binding Proteins
• Fibroblasts (microbiology)
• Interferons (pharmacology)
• Protein Binding
• Vaccinia virus (metabolism)
• Viral Proteins (biosynthesis, isolation & purification)