Abstract |
Hybridomas secreting monoclonal antibodies directed against polyprotein gene products of the Gardner, Snyder-Theilen, and McDonough strain of feline sarcoma virus have been isolated. Antibody produced by one hybridoma recognizes immunological determinants localized within a feline leukemia virus gag gene structural component (p15) common to polyproteins encoded by each feline sarcoma virus isolate while antibody produced by a second is specific for p30 determinants unique to P170gag-fms. Additional hybridomas secrete antibody directed against v-fes specific determinants common to the Gardner and Snyder-Theilen feline sarcoma virus-encoded polyproteins and to v-fms determinants unique to P170gas-fms polyprotein. GA P110gas-fes and ST P85gas-fes immunoprecipitated by antibody directed against p15 exhibit readily detectable levels of protein kinase activity but lack such activity when precipitated by antibody specific for their acquired sequence (v-fes) components. P170gas-fms immunoprecipitated by monoclonal antibody to either p15 or p30 lacks detectable levels of autophosphorylation but represents a substrate for the GA P110gag-fes and ST P85gag-fes enzymatic activities. These findings argue that the v-fes-associated protein kinase represents an intrinsic property of the v-fes gene product and recognizes tyrosine acceptor sites within polyprotein gene products of all three strains of feline sarcoma virus.
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Authors | F Veronese, G J Kelloff, F H Reynolds Jr, R W Hill, J R Stephenson |
Journal | Journal of virology
(J Virol)
Vol. 43
Issue 3
Pg. 896-904
(Sep 1982)
ISSN: 0022-538X [Print] United States |
PMID | 6183442
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Antibodies, Viral
- Viral Proteins
- Phosphotyrosine
- Tyrosine
- Protein Kinases
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Topics |
- Animals
- Antibodies, Monoclonal
(isolation & purification)
- Antibodies, Viral
(isolation & purification)
- Ascitic Fluid
(immunology)
- Hybridomas
(immunology)
- Leukemia Virus, Feline
(immunology)
- Phosphotyrosine
- Protein Kinases
(metabolism)
- Rats
- Rats, Inbred F344
- Retroviridae
(immunology)
- Sarcoma Viruses, Feline
(immunology)
- Tyrosine
(analogs & derivatives, analysis)
- Viral Proteins
(immunology)
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