Abstract |
Dorf and colleagues (1-4) found that the contact sensitivity (CS) primed with (4-hydroxy-3-nitrophenyl)acetyl (NP) could be elicited as easily with the iodoanalog (NIP) as with NP when studied in Igh-1b mice but could only be elicited with NP, not NIP, in Igh-1j mice. Since this fine-specificity was parallel to the fine-specificity of anti-NP antibodies in the two types of mice and since anti-NP antibodies of Igh-1b mice are controlled by gene Igh- NPb the authors concluded that CS also was controlled by the Igh- NPb gene. The aim of this study was to confirm their findings with a more quantitative method (5). We confirmed equality of NP and NIP as elicitors of NP-primed CS in Igh-1b mice when the priming antigen was given subcutaneously into non- cyclophosphamide-treated mice (their method). We also found that this priming induced an anti-NP antibody response detectable at the time of challenge. Most experiments were carried out with a method that does not induce a detectable antibody response (pretreatment of mice with 200 mg/kg of cyclophosphamide; application of the sensitizing compound on skin). Since the NP-primed (and NBrP-primed) CS reactions exhibited "expected specificities," the immunizing compound was clearly the most efficient elicitor (relative efficiencies of homologs varied from 2 to 4). The Igh- NPb gene appears not to have a role in "antibody-free" reactions.
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Authors | A Matoso-Ferreira, O Mäkelä |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 130
Issue 1
Pg. 97-101
(Jan 1983)
ISSN: 0022-1767 [Print] United States |
PMID | 6183362
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Epitopes
- Nitrophenols
- Phenylacetates
- 4-hydroxy-5-nitrophenyl acetic acid
- Cyclophosphamide
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Topics |
- Animals
- Cross Reactions
- Cyclophosphamide
(pharmacology)
- Dermatitis, Contact
(immunology)
- Epitopes
- Hypersensitivity, Delayed
(immunology)
- Immunity, Cellular
(drug effects)
- Mice
- Nitrophenols
(immunology)
- Phenylacetates
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