Use of monoclonal antibodies in detection of melanoma-associated antigens in intact human tumors.

Abstract

The use of antimelanoma monoclonal antibodies on tissue sections using a two-step indirect immunoperoxidase technique is reported. Antibodies 691-13-17 and 691-I5-Nu4B reacted with dysplastic nevus cells and all melanomas tested, but not with normal skin melanocytes, intradermal nevi, or lentigines. Antibody 691-13-17, directed against DR antigen, reacted also with Langerhan's cells, macrophages, and a subpopulation of lymphocytes. Antibody 691-I5-Nu4B reacted only with melanomas. The technique allows analysis of the expression of antigens by tumor cells in situ.

• Authors: J J Thompson, M F Herlyn, D E Elder, W H Clark, Z Steplewski, H Koprowski
• Journal: The American journal of pathology (Am J Pathol) Vol. 107 Issue 3 Pg. 357-61 (Jun 1982) ISSN: 0002-9440 [Print] United States
• PMID: 6177248 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Neoplasm

Topics

• Adult
• Aged
• Antibodies, Monoclonal (immunology)
• Antibody Specificity
• Antigens, Neoplasm (analysis)
• Female
• Humans
• Immunoenzyme Techniques
• Lymphatic Metastasis
• Male
• Melanoma (diagnosis, immunology)
• Middle Aged
• Skin Neoplasms (diagnosis, immunology)
• Staining and Labeling