Abstract |
Murine keratinocytes, isolated by flotation trypsinization of skin, can be separated into five groups by centrifugation through Percoll, a colloidal silica gradient. Within each group a good correlation was found between density, plating efficiency, morphological appearance, DNA synthesis, and degree of keratinization/cornification. This method can be applied equally well to fetal, newborn, or adult keratinocytes and should be useful in a variety of studies including isolation of subpopulations of pathological cell types, work on chalones and hyperplastic diseases such as psoriasis, and in vitro transformation studies.
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Authors | S M Fischer, K D Nelson, J J Reiners Jr, A Viaje, J C Pelling, T J Slaga |
Journal | Journal of cutaneous pathology
(J Cutan Pathol)
Vol. 9
Issue 1
Pg. 43-9
(Feb 1982)
ISSN: 0303-6987 [Print] United States |
PMID | 6175674
(Publication Type: Journal Article)
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Chemical References |
- Percoll
- Keratins
- Silicon Dioxide
- DNA
- Povidone
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Topics |
- Animals
- Cell Differentiation
- Cell Transformation, Neoplastic
(pathology)
- Centrifugation, Density Gradient
(methods)
- DNA
(biosynthesis)
- Epidermal Cells
- Keratins
(analysis)
- Mice
- Povidone
- Silicon Dioxide
- Skin
(cytology)
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