Separation of epidermal cells by density centrifugation: a new technique for studies on normal and pathological differentiation.

Abstract

Murine keratinocytes, isolated by flotation trypsinization of skin, can be separated into five groups by centrifugation through Percoll, a colloidal silica gradient. Within each group a good correlation was found between density, plating efficiency, morphological appearance, DNA synthesis, and degree of keratinization/cornification. This method can be applied equally well to fetal, newborn, or adult keratinocytes and should be useful in a variety of studies including isolation of subpopulations of pathological cell types, work on chalones and hyperplastic diseases such as psoriasis, and in vitro transformation studies.

• Authors: S M Fischer, K D Nelson, J J Reiners Jr, A Viaje, J C Pelling, T J Slaga
• Journal: Journal of cutaneous pathology (J Cutan Pathol) Vol. 9 Issue 1 Pg. 43-9 (Feb 1982) ISSN: 0303-6987 [Print] United States
• PMID: 6175674 (Publication Type: Journal Article)

Chemical References

• Percoll
• Keratins
• Silicon Dioxide
• DNA
• Povidone

Topics

• Animals
• Cell Differentiation
• Cell Transformation, Neoplastic (pathology)
• Centrifugation, Density Gradient (methods)
• DNA (biosynthesis)
• Epidermal Cells
• Keratins (analysis)
• Mice
• Povidone
• Silicon Dioxide
• Skin (cytology)