A protein fraction (NHG) from serum of normal humans which is cytotoxic for HeLa cells in culture.

Abstract

A fraction of the alpha-globulins (NHG) from normal human serum was cytotoxic for mouse L-cells in culture and Meth A tumors in mice. NHG inhibited the growth in vitro of human colon cancer (HT-29), melanoma (RPMI 7931) and a neuroblastoma cell line. Survival of HeLa S-3 cell colonies after 24 h exposure to 25, 50, 75 or 100 micrograms NHG/ml medium was 86%, 77%, 40% and 10%, respectively. Whole human serum or purified serum albumin had no anti-HeLa cell activity. These results confirm the presence of a protein in human serum with antitumor activity. An assay for NHG using HeLa S-3 tumor cells is described.

• Authors: S Green, S H Kim, J Hlinka, M A Chiasson, A Dobrjansky, N A Ensign
• Journal: Cancer letters (Cancer Lett) Vol. 11 Issue 4 Pg. 345-50 (Feb 1981) ISSN: 0304-3835 [Print] Ireland
• PMID: 6170426 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Alpha-Globulins
• Antineoplastic Agents

Topics

• Alpha-Globulins (isolation & purification, pharmacology)
• Antineoplastic Agents (isolation & purification, pharmacology)
• HeLa Cells (drug effects)
• Humans
• Molecular Weight