High mobility group chromosomal proteins isolated from muclei and cytosol of cultured hepatoma cells are similar.

Using sequential chromatography on columns containing immobilized double-stranded DNA and single-stranded DNA, we have purified a protein from the cytosol of an established line of cultured rat hepatoma cells that, by several criteria, is a high mobility group (HMG) protein. Analyses of DNA binding properties, electrophoretic mobilities, amino acid compositions, and immunochemical reactivities reveal that the cytosolic protein is the same protein as HMG-1 isolated from the purified chromatin of the same cell line. Thus, authentic HMG-1 appears to be at least partially responsible for the cytoplasmic fluorescence observed when mammalian cells are stained with fluorescece observed when mammalian cells are stained with fluorescent-labeled, affinity-purified antibodies against HMG-1 [Bustin, M., & Neihart, N.K. (1979) Cell 16, 181-189]. We suggest that HMG-1 cn shuttle between nucleus and cytoplasm, perhaps in response to the nucleus' need for helix destabilizing proteins.
AuthorsP J Isackson, D L Bidney, G R Reeck, N K Neihart, M Bustin
JournalBiochemistry (Biochemistry) Vol. 19 Issue 19 Pg. 4466-71 (Sep 16 1980) ISSN: 0006-2960 [Print] UNITED STATES
PMID6157409 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Amino Acids
  • Chromosomal Proteins, Non-Histone
  • Epitopes
  • High Mobility Group Proteins
  • DNA
  • Amino Acids (analysis)
  • Animals
  • Cattle
  • Cell Line
  • Cell Nucleus (analysis)
  • Chromatography, Affinity
  • Chromosomal Proteins, Non-Histone (isolation & purification)
  • Complement Fixation Tests
  • Cytosol (analysis)
  • DNA
  • Epitopes
  • High Mobility Group Proteins
  • Humans
  • Liver Neoplasms, Experimental (analysis)
  • Molecular Weight
  • Rats
  • Thymus Gland

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