Abstract |
The terminal differentiation, keratinization, of a rat bladder tumor cell line, NBT II, occurred in multicellular aggregates. After aggregation, these cells did not undergo a round of mitosis before keratinization. 5-Bromodeoxyuridine added to the monolayer cell culture 2 days before aggregation completely prevented this differentiation; it was ineffective when added at the time of cell aggregation. Vitamin A prevented the keratinization of NBT II cells in aggregates but did not inhibit aggregate formation; it enhanced the number of cells engaged in DNA synthesis. This model appears to be very useful for analyzing the mechanisms of terminal differentiation and its modulation by vitamin A in tumor cells.
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Authors | R Tchao |
Journal | In vitro
(In Vitro)
Vol. 16
Issue 5
Pg. 407-14
(May 1980)
ISSN: 0073-5655 [Print] United States |
PMID | 6156120
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Neoplasm
- Cytarabine
- Vitamin A
- Keratins
- Bromodeoxyuridine
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Topics |
- Animals
- Bromodeoxyuridine
(pharmacology)
- Carcinoma, Squamous Cell
- Cell Aggregation
(drug effects)
- Cell Differentiation
(drug effects)
- Cell Line
- Cytarabine
(pharmacology)
- DNA, Neoplasm
(biosynthesis)
- Keratins
(metabolism)
- Mitosis
- Rats
- Urinary Bladder Neoplasms
- Vitamin A
(pharmacology)
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