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Keratinization and the effect of vitamin A in aggregates of a squamous carcinoma cell line NBT II.

Abstract
The terminal differentiation, keratinization, of a rat bladder tumor cell line, NBT II, occurred in multicellular aggregates. After aggregation, these cells did not undergo a round of mitosis before keratinization. 5-Bromodeoxyuridine added to the monolayer cell culture 2 days before aggregation completely prevented this differentiation; it was ineffective when added at the time of cell aggregation. Vitamin A prevented the keratinization of NBT II cells in aggregates but did not inhibit aggregate formation; it enhanced the number of cells engaged in DNA synthesis. This model appears to be very useful for analyzing the mechanisms of terminal differentiation and its modulation by vitamin A in tumor cells.
AuthorsR Tchao
JournalIn vitro (In Vitro) Vol. 16 Issue 5 Pg. 407-14 (May 1980) ISSN: 0073-5655 [Print] United States
PMID6156120 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Neoplasm
  • Cytarabine
  • Vitamin A
  • Keratins
  • Bromodeoxyuridine
Topics
  • Animals
  • Bromodeoxyuridine (pharmacology)
  • Carcinoma, Squamous Cell
  • Cell Aggregation (drug effects)
  • Cell Differentiation (drug effects)
  • Cell Line
  • Cytarabine (pharmacology)
  • DNA, Neoplasm (biosynthesis)
  • Keratins (metabolism)
  • Mitosis
  • Rats
  • Urinary Bladder Neoplasms
  • Vitamin A (pharmacology)

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