Effects of 2',3'-dideoxythymidine triphosphate on replicative DNA synthesis and unscheduled DNA synthesis in permeable mouse sarcoma cells.

Abstract

2',3'-Dideoxythymidine triphosphate differentially inhibited replicative DNA synthesis in permeable mouse ascites sarcoma cells and unscheduled DNA synthesis in bleomycin-treated permeable cells or in isolated rat liver nuclei. The mode of inhibition of 2',3'-dideoxythymidine triphosphate was competitive with respect to deoxythymidine triphosphate. 2',3'-Dideoxythymidine triphosphate inhibited replicative DNA synthesis with a Ki of 8 microM, whereas unscheduled DNA synthesis was more sensitive, the Ki being 0.5 microM. Referring to the differential sensitivity of DNA polymerases alpha and beta to 2',3'-dideoxythymidine triphosphate and to other related information reported previously, the present results suggested that DNA polymerase alpha is playing a major role in replicative DNA synthesis, and DNA polymerase beta in unscheduled DNA synthesis.

• Authors: S Seki, T Oda
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 606 Issue 2 Pg. 246-50 (Feb 29 1980) ISSN: 0006-3002 [Print] Netherlands
• PMID: 6153534 (Publication Type: Journal Article)

Chemical References

• DNA, Neoplasm
• Dideoxynucleotides
• Thymine Nucleotides
• Bleomycin
• DNA-Directed DNA Polymerase
• 2',3'-dideoxythymidine triphosphate

Topics

• Animals
• Bleomycin (pharmacology)
• Cell Membrane Permeability (drug effects)
• DNA Replication (drug effects)
• DNA, Neoplasm (biosynthesis)
• DNA-Directed DNA Polymerase (metabolism)
• Dideoxynucleotides
• Mice
• Sarcoma, Experimental (metabolism)
• Thymine Nucleotides (pharmacology)