Conversion of 14C-labeled eicosapentaenoic acid (n-3) to leukotriene C5.

Abstract

14C-labeled eicosapentaenoic acid (n-3) was converted by mouse mastocytoma cells to 5-hydroxy-6-S-glutathionyl-7,9,11,14,17-eicosapentaenoic acid (leukotriene C5). The identification was based on comparisons with previously characterized unlabeled material by high-performance liquid chromatography, ultraviolet spectroscopy, and conversion by gamma-glutamyl transpeptidase to leukotriene D5.

• Authors: S Hammarström
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 663 Issue 2 Pg. 575-7 (Feb 23 1981) ISSN: 0006-3002 [Print] Netherlands
• PMID: 6111355 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fatty Acids, Unsaturated
• SRS-A
• leukotriene C-5
• Eicosapentaenoic Acid
• gamma-Glutamyltransferase

Topics

• Animals
• Chromatography, High Pressure Liquid
• Eicosapentaenoic Acid
• Fatty Acids, Unsaturated (metabolism)
• Mast-Cell Sarcoma (metabolism)
• Mice
• SRS-A (analogs & derivatives, metabolism)
• Sarcoma, Experimental (metabolism)
• Spectrophotometry, Ultraviolet
• gamma-Glutamyltransferase (metabolism)