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Poly(A)-mRNA deprimerones in rat liver and Novikoff hepatoma cells.

Abstract
The poly (A)-mRNA fraction isolated by chloroform deproteinization of liver polysomes and poly(U)-Sepharose chromatography contains a low molecular weights (congruent to 1000) peptidic fraction. The peptides which we suggested to call deprimerones (1) were extracted with 80% ethanol at pH 9.5; after ethanol evaporation, they were purified on Sephadex G-25 column as a fraction of mol. wt. between 1600 and 600, yielding about 9 mg/mg mRNA. If deproteinization is performed with phenol-chloroform the yield is about 2 mg/mg mRNA. In Novikoff hepatoma the yield of the same preparation is only 2.7 mg/mg mRNA (about 70% decrease). The obtained deprimerones are active in inhibiting transcription of thymus DNA with E. coli RNA polymerase and [3H]-GTP by about 90% at a ratio peptide/DNA = 2. For comparison the deprimerones obtained previously (2) by extraction of deproteinized DNA inhibit transcription only by about 50% at the same peptide/DNA ratio. The results demonstrated a decrease of the poly (A)-mRNA deprimerone level during carcinogenesis and further support the previously demonstrated specific occurrence of deprimerones with poly(A)-mRNA. They remain in accordance with and provide further support for the deprimerone theory of carcinogenesis postulated earlier (1).
AuthorsM Hiliar, J Przyjemski, Z Stolzmann
JournalMolecular biology reports (Mol Biol Rep) Vol. 6 Issue 2 Pg. 89-94 (Jul 31 1980) ISSN: 0301-4851 [Print] Netherlands
PMID6106157 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Peptides
  • RNA, Messenger
  • deprimerones
  • Poly A
  • Peptide Hydrolases
  • Subtilisins
Topics
  • Animals
  • Liver (metabolism)
  • Liver Neoplasms, Experimental (metabolism)
  • Molecular Weight
  • Peptide Hydrolases (metabolism)
  • Peptides (metabolism, pharmacology)
  • Poly A (metabolism)
  • RNA, Messenger (metabolism)
  • Rats
  • Subtilisins
  • Swine
  • Transcription, Genetic (drug effects)

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